JMIR Publications

ABSTRACT

The novel coronavirus has a high mortality rate (over 1% for patients older than 50). This could be only partially ascribed to other comorbidities. Possible explanation could be something which assures the ability to prompt response to SARS-CoV-2 in younger people independently from the novelty of the virus itself. Something stimulated the immune system and it scattered immunity against more antigens. The only external stimulation, which healthy people receive, is vaccination (i.e. diphtheria, tetanus, and pertussis (DTP) vaccine). One hypothesis is that vaccination develops the specific immunity but generates a sprouting immunity against antigens in transit. The underlying immunological phenomena are “bystander effect” and “trained immunity”. The developed immunity gives protection for years until the natural fade out. After the fifth decade a viral infection will find immune system almost incompetent and the novel coronavirus bursts into the body, developing an ARDS. The first study’s aim is to demonstrate that monocytes, NK, CD4 + and CD8 + T cells, in patients with severe infection to SARS-CoV-2, show an overpowering hyperactivity. The secondary objectives are to correlate clinical data and vaccination history with laboratory immune pattern, to identify protective factors. Four categories of 30 patients will be analyzed: A) Asymptomatic patients; B) Mildly symptomatic patients: with fever, tiredness, dry cough, diarrhea, ect; C) Patients with diagnosis of pneumonia with “low risk” score; D) Patients with diagnosis of pneumonia with “moderate/high” risk score. Data will be collected using 3 approaches: An experimental analysis to study the innate immune response and to identify the genetic profiles; An epidemiological analysis to identify the patients’ vaccination history; A clinical analysis to detect the immunological profile. We suppose to recognize different populations of patients, each one with a specific immunological pattern in terms of cytokines, soluble factors serum level and immune cells activity. Anamnestic data such as preceding vaccinations and comorbidities, biochemical data findings as lymphocyte immunophenotyping and pre-existing persistent cytomegalovirus infection allow depicting the risk profile of severe COVID-19. The proof of a role of these immunological phenomena on the development of COVID-19 are bases for implementation of therapeutic immunomodulatory treatments.