Accepted for/Published in: JMIR Research Protocols
Date Submitted: Jul 11, 2018
Open Peer Review Period: Jul 12, 2018 - Jul 26, 2018
Date Accepted: Mar 24, 2019
(closed for review but you can still tweet)
Diet-Induced Alteration of Microbiota and development of Obesity, Nonalcoholic fatty liver disease and Diabetes (DIAMOND): study protocol of a prospective study
ABSTRACT
Background:
Development of obesity and obesity related diseases such as type 2 diabetes mellitus and nonalcoholic fatty liver disease is associated with altered gut microbiota composition. The aim of this study is to investigate associations between dietary compounds, intestinal cell function and gut microbiota composition. We hypothesize that dietary lipid intake is associated with Paneth cell and goblet cell properties that affect gut microbiota composition.
Objective:
The primary objective of this study is to determine whether a difference in dietary intake is associated with a difference in intestinal mucin-2 expression and gut microbiota composition.
Methods:
This is a single-centre prospective study including one obese group undergoing laparoscopic roux-en-y gastric bypass and two lean control groups undergoing either laparoscopic cholecystectomy or upper gastrointestinal endoscopy (n=228). During laparoscopy, biopsies will be taken of visceral fat (omentum majus), liver, muscle tissue of the abdominal wall, and subcutaneous fat. In the obese group, a small segment of the jejunum will be collected for analysis, which will be compared with an endoscopically derived jejunal biopsy from the upper gastrointestinal endoscopy control group. Stool samples for microbiota profiling will be collected at baseline and one year after surgery. Primary outcomes are fecal microbiota composition and mucus characteristics. Secondary outcomes include Paneth cell phenotype, body weight, diet composition, glucose tolerance,resolution of comorbidities and weight loss one year after surgery.
Results:
This trial is currently open for recruitment. The anticipated completion date is December 2019.
Conclusions:
The DIAMOND-study will improve insight into the pathophysiology of obesity and its associated metabolic disorders. Better understanding of weight loss failure and weight regain following bariatric surgery might also behold new therapeutic opportunities for obesity and obesity-related comorbidities. Clinical Trial: Nederlands Trial Register: NTR5660
Citation
Per the author's request the PDF is not available.
Copyright
© The authors. All rights reserved. This is a privileged document currently under peer-review/community review (or an accepted/rejected manuscript). Authors have provided JMIR Publications with an exclusive license to publish this preprint on it's website for review and ahead-of-print citation purposes only. While the final peer-reviewed paper may be licensed under a cc-by license on publication, at this stage authors and publisher expressively prohibit redistribution of this draft paper other than for review purposes.