JMIR Publications

ABSTRACT

Background:

Insulin resistance, altered glucose metabolism, and decreased insulin production are all features of type 2 diabetes mellitus (T2DM), a complicated metabolic illness. Despite the fact that traditional anti-diabetic medications successfully lower blood sugar, long-term use of these medications is linked to side effects and has no effect on underlying disease. Ayurveda describes Prameha and Madhumeha as conditions analogous to diabetes and advocates herbomineral formulations processed through pharmaceutical techniques such as Bhavana to enhance therapeutic efficacy and bioavailability.

Objective:

To perform pharmaceutical and analytical evaluation of a novel herbomineral formulation and investigate its mechanistic antidiabetic activity through in vitro studies.

Methods:

From October 2026 to October 2027, a pharmaceutical analytical and in-vitro experimental investigation will be carried out to prepare pharmaceuticals and assess the mechanistic anti-diabetic activity of a novel herbomineral formulation. At the Vedpurna Ayurved Rasashala, Mahatma Gandhi Ayurved College, Hospital and Research Centre, Salod (H), Wardha, the formulation will be prepared using traditional Ayurvedic methods; advanced analytical and in-vitro studies will be conducted at AIHER (DU) accredited laboratories/institutes. The prepared formulation will undergo traditional Bhasma Pariksha, organoleptic and physicochemical evaluation, along with advanced characterization using EDAX, SEM, FTIR, and XRD techniques. The in-vitro anti-diabetic activity will be assessed using 3T3-L1 adipocyte cell lines, as they are widely accepted for evaluating insulin sensitivity and glucose uptake in Type 2 Diabetes Mellitus. 3T3-L1 adipocytes for evaluation of glucose uptake/insulin sensitivity-related activity. MIN6 mouse pancreatic β-cells for evaluation of insulin-secretory activity MTT cytotoxicity assay, insulin stimulation assay, and glucose uptake assay will be performed in control, standard, and test groups with experiments carried out in triplicate. The formulation is selected based on the synergistic anti-diabetic potential of its ingredients, including insulin-sensitizing, antioxidant, and glucose-regulating activities. Statistical analysis will be performed using One-way ANOVA and Student’s t-test, and a p-value of <0.05 will be considered statistically significant.

Results:

It is anticipated that the formulation will exhibit considerable α-amylase inhibitory action, better insulin secretion, and increased glucose absorption.

Conclusions:

Preliminary mechanistic evidence for the creation of a novel Ayurvedic herbomineral formulation for the treatment of diabetes may be found in this work.