Accepted for/Published in: JMIR Research Protocols
Date Submitted: Sep 6, 2024
Date Accepted: May 23, 2025
Warning: This is an author submission that is not peer-reviewed or edited. Preprints - unless they show as "accepted" - should not be relied on to guide clinical practice or health-related behavior and should not be reported in news media as established information.
Study protocol: Investigating the role of Coenzyme A restriction in the pathophysiology of Pre-eclampsia
ABSTRACT
Background:
Pre-eclampsia is one of the three leading causes of maternal mortality worldwide. Un-fortunately, the exact pathogenesis is still unclear. Published metabolomic and gene expression analyses point to Coenzyme A restriction in the placenta as a factor under-pinning the observed complications of pre-eclampsia, but this hypothesis has never been tested.
Objective:
Investigate the role of CoA restriction in pre-eclampsia
Methods:
This manuscript provides a rationale and a protocol for a clinical study and laboratory experiments to test this hypothesis. It involves screening pre-eclamptic patients for elevated levels of key toxic metabolites induced by the restriction. The laboratory study will involve treating cultured trophoblast cells in a variety of ways to determine if Co-enzyme A restriction can be induced. It involves both metabolite profiling of the culture medium for the toxic metabolites and expression profiling of key genes.
Results:
Patients are currently being recruited into the clinical study, and preliminary results will be published in 2026.
Conclusions:
The findings of this study will indicate if Coenzyme A restriction is associated with pre-eclampsia. If so, this provides a significant novel avenue for research into the treatment and prevention of the disease. Clinical Trial: Not applicable
Citation
Request queued. Please wait while the file is being generated. It may take some time.
Copyright
© The authors. All rights reserved. This is a privileged document currently under peer-review/community review (or an accepted/rejected manuscript). Authors have provided JMIR Publications with an exclusive license to publish this preprint on it's website for review and ahead-of-print citation purposes only. While the final peer-reviewed paper may be licensed under a cc-by license on publication, at this stage authors and publisher expressively prohibit redistribution of this draft paper other than for review purposes.