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Accepted for/Published in: JMIR Research Protocols

Date Submitted: Aug 9, 2024
Date Accepted: Jun 18, 2025

The final, peer-reviewed published version of this preprint can be found here:

Kissing as a Protective Factor Against Decreased Salivary pH: Protocol for a Randomized Clinical Trial

Briones FM Sr, Bermúdez Velásquez A Sr, Aguila Gaibor M

Kissing as a Protective Factor Against Decreased Salivary pH: Protocol for a Randomized Clinical Trial

JMIR Res Protoc 2025;14:e65253

DOI: 10.2196/65253

PMID: 40674724

PMCID: 12314465

Warning: This is an author submission that is not peer-reviewed or edited. Preprints - unless they show as "accepted" - should not be relied on to guide clinical practice or health-related behavior and should not be reported in news media as established information.

Kissing as a protective factor against decreased salivary pH. A randomized controlled trial

  • Fernando Marcelo Briones Sr; 
  • Andrea Bermúdez Velásquez Sr; 
  • Mariuxi Aguila Gaibor

ABSTRACT

Background:

Kissing is a common social behavior that may influence physiological responses and impact oral health. Prior research has shown that affectionate behaviors like kissing can reduce stress and improve relationship satisfaction. However, the effects of kissing on salivary pH, particularly after consuming sugary or fermented drinks, have not been thoroughly investigated. This study aims to explore whether kissing can neutralize the acidic pH of saliva caused by such beverages.

Objective:

To determine the effect of kissing on salivary pH following the consumption of sugary or fermented drinks.

Methods:

A randomized controlled trial (RCT) was conducted with 60 couples aged 18-30. Participants were divided into three groups: a control group (no kissing), an experimental group where one partner consumed a drink, and another experimental group where both partners consumed a drink. The study measured salivary pH at baseline, after drink consumption, and following a 40-second kiss or a control period, with measurements taken every 5 minutes until pH returned to neutral levels. Data were analyzed using SPSS software, employing t-tests, ANOVA, and other relevant statistical methods.

Results:

The study is ongoing, with preliminary data expected in November 2024. Initial recruitment indicates sufficient interest from the target population.

Conclusions:

This study will provide insights into the relationship between kissing, salivary pH, and oral health, potentially offering new strategies for dental caries prevention. The results may challenge existing assumptions about the role of kissing in maintaining oral hygiene. Further research will be needed to confirm these findings. Clinical Trial: ClinicalTrials.gov NCT06501729


 Citation

Please cite as:

Briones FM Sr, Bermúdez Velásquez A Sr, Aguila Gaibor M

Kissing as a Protective Factor Against Decreased Salivary pH: Protocol for a Randomized Clinical Trial

JMIR Res Protoc 2025;14:e65253

DOI: 10.2196/65253

PMID: 40674724

PMCID: 12314465

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