Accepted for/Published in: JMIR Research Protocols
Date Submitted: Jul 17, 2024
Open Peer Review Period: Jul 21, 2024 - Sep 15, 2024
Date Accepted: Apr 4, 2025
(closed for review but you can still tweet)
Warning: This is an author submission that is not peer-reviewed or edited. Preprints - unless they show as "accepted" - should not be relied on to guide clinical practice or health-related behavior and should not be reported in news media as established information.
Assessing the Role of Blood Group Genotyping in Predicting Hemolysis in Thalassemia Beta Major Patients with Repeated Transfusions: Study Protocol
ABSTRACT
Background:
Hemolytic transfusion reactions are a major complication in patients with thalassemia beta major receiving regular transfusions. Blood group compatibility may play a major role in the incidence of these reactions. However, there is a lack of studies in Indonesia assessing the role of blood group genotyping in hemolytic transfusion.
Objective:
This study aims to analyze the association between blood group genotyping and the incidence of hemolysis in thalassemia patients undergoing repeated transfusions.
Methods:
This is a cross-sectional study involving patients with thalassemia beta major under 18 years old who received regular transfusion with intervals of 2-4 weeks and have received more than 20 units of transfusion. Subjects with leukemia, lymphoproliferative diseases, diabetes, solid tumors, and immunosuppression disorders were excluded from the study. Genotyping examination was conducted using PCR ASP while phenotyping was examined using immunoserology. Follow-up gene sequencing was conducted to observe the blood group variants. Hemolysis was assessed using several markers such as haptoglobulin, free hemoglobin, LDH, bilirubin, hemoglobinurin, measured by Cobas C113, ELISA and urinalysis.
Results:
Clinical and laboratory data is completed. A total of 134 samples were collected, data analyses are undertaken, and the first results reporting findings is expected to be in July 2024
Conclusions:
The results of this study will provide information on blood groups system that can predict hemolysis occurrence in thalassemia beta major patients with repeated transfusion. This data will contribute to the best possible patient care management and blood transfusion therapy, thereby reducing the risk of hemolysis and improving the quality of life for thalassemia patients in Indonesia. Clinical Trial: -
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