Previously submitted to: JMIR Bioinformatics and Biotechnology (no longer under consideration since Jun 21, 2024)
Date Submitted: Apr 12, 2024
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Preparation, characterization of Selenium nanoparticles from E. coli and study its effect on pathogenic bacteria.
ABSTRACT
Bacterial infections might be regarded as one of the primary issues confronting the healthcare system at the moment. The development of nanoparticles offers an alternative to the usage of antibiotics. Sixty five isolates of Escherichia coli were isolated from various specimens, and used for the production of selenium nanoparticles at concentration 100 mM during 48 h, pH 8, agitation speed 160 rpm, and visualized by appearance of red color in the medium. Characterization employing UV-Vis spectroscopy showed absorption band formed at 266 nm, while, in XRD the Se-NPs peaks centered at 2θ of 27.605 , 32.092, 45.652, 56.815, 66.458 and 75.632, corresponded to the crystal planes of (100), (101), (012), (200),(022) and (210). Zeta potential demonstrate the colloidal dispersion's stability of Se-NPs with negative charge (-6mV), and SEM showed spherically-shaped NPs with a size range between (46.71–71.91). Antimicrobial activity showed the highest inhibition zones to Klebsiella pneumoniae and Staphylococcus aureus (19.67 and 18.50) mm at 50 µl/ml respectively. The maximum antibiofilm activity of SeNPs was observed to be against S. aureus, P. aeruginosa, Klebsiella pneumoniae and E. coli at Sub MIC concentration were (80.81%,75.35 %, 64.67% and 65.41%). So, it can be used as an effective novel antimicrobial and antibiofilm agent.
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