Previously submitted to: JMIR Bioinformatics and Biotechnology (no longer under consideration since Mar 01, 2022)
Date Submitted: Mar 25, 2021
Open Peer Review Period: Mar 25, 2021 - May 20, 2021
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Discovery and Topic Evolution Analysis of CRISPR/Cas9 Gene Editing Technology in Biomedical Field:Internet-based Survey
ABSTRACT
Background:
In 2020, the discoverers of CRISPR/Cas9 technology, Jennifer A. Doudna and Emmanuelle Charpentier, were awarded the Nobel Prize in Chemistry. This technology has revolutionized the field of biomedical science since its birth and contributed to the treatment of a variety of complex diseases. Scholars have carried out many studies on the development trend of CRISPR technology, bibliometrics, visualization and other aspects. However, there are few studies on the hot spot analysis and topic changes in the biomedical field, which is not conducive to scholars' thinking.
Objective:
This study aimed to:(1) mining and analyzing the research hotspots of CRISPR/Cas9 system in each stage; (2) sorting out and visualizing the evolution direction of the hot research topics of CRISPR/Cas9 gene editing technology in the past decade; (3) exploring the hidden research hotspots of CRISPR/Cas9 gene editing technology, so as to provide reference for further research
Methods:
In this study, research papers related to CRISPR/Cas9 gene editing technology in biomedical field published on the Web of Science core datasets from 2010 to 2020 were used; literature research method, analysis method and expert consultation method and other methods were comprehensively utilized; CiteSpace software and VOS viewer software were selected as visualization tools to analyze hot topics and topic evolution in research.
Results:
The generated co-occurrence map and cluster map were analyzed by keyword analysis strategy to determine the hot topics in each development stage. The topic evolution types and evolution paths are studied based on the categories and keywords of hot topics in each stage and the characteristics of their advancement along the time axis, and the topic evolution diagram and evolution path diagram were presented respectively.
Conclusions:
Based on the above research results, firstly, in recent years, scholars have attached great importance to the research on this technology in the field of biomedical science, and the research topics have been deepened and enriched. Secondly, the development of CRISPR/Cas9 system shows different hot research directions in different periods, which is in line with the development of The Times. Finally, the theme evolution of CRISPR/Cas9 in the field of biomedicine generally shows a trend from single to multiple, and from theory to practice. Hidden research hotspots focus on the detection and treatment of infectious diseases, aging mitigation, drug resistance and other common diseases.
Citation
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