Previously submitted to: JMIR Medical Informatics (no longer under consideration since Jun 23, 2021)
Date Submitted: Apr 5, 2020
Improving the Rehabilitation Treatment of Staphylococcus aureus Infection in Older Adults With Bone and Joint Diseases: Analysis Using RNAIII-Inhibiting Peptide and an Image Information Counting Algorithm
Background:
Due to the prevalence of multiple joint problems among older adults, joint diseases are a source of inconvenience that reduces quality of life in this population.
Objective:
Our study aims to explore the application of RNAIII-inhibiting peptide (RIP) and an image information counting algorithm in the rehabilitation treatment plan of Staphylococcus aureus (S. aureus) infection in the bones and joints of older adults.
Methods:
The relationship between the ability of S. aureus to form biofilms and the concentration of RIP was observed. Additionally, the influence of RIP on the adhesion of S. aureus was counted and analyzed by an image information counting algorithm. The initial image of S. aureus was processed in grayscale, then subjected to expansion operation, and the processed image was transformed into a binary image for counting. Real-time quantitative polymerase chain reaction (RT-q PCR) was used to detect the mRNA (messenger RNA) levels of five genes related to biofilm formation—ica A, ica R, fnb A, atl A, and sar A.
Results:
The results show that the expression level of the ica A, ica R, fnb A, and atl A genes in the experimental group was significantly lower than that of the control group, and the expression level of ica R in the experimental group was significantly higher than that of the control group (P<.05). There was no significant difference between the two groups in the gene expression of sar A. Through the image information counting algorithm, it was found that RIP could inhibit the adhesion of S. aureus and the formation of early biofilm, but the specific regulatory mechanism needs further study.
Conclusions:
This study suggests that RIP is applicable to the treatment of elderly patients with bone and joint diseases caused by S. aureus infection (eg, pyogenic arthritis and osteomyelitis). Our experiment may prompt new ideas for the treatment of diseases caused by S. aureus infection.
Citation
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